HONG KONG- Several moths ago Oscar, the graduate student I am working with, acclimated four Japanese eels (Anguilla japonica) in one of four hyposmotic environments.
1) deionized water (DI)
2) DI and NaCl
3) DI and Na+
4) DI and Cl-
Then dissected and removed kidney tissue, which was stored in the freezer. Last week we extracted a subset of sequence of DNA, from those samples that codes for stanniocalcin (STC). This hormone it responsible for Ca2+ regulation, but could possibly affect Na- and Cl+ too. Once the DNA was amplified it was electrophorised, to determine if the gene was present.